Growth and yield of Capsicum Annum Under Irrigation with Different Levels of Domestic Waste Water
1
Division of Soil Sciences,
S.K. University of Agricultural Sciences and Technology of Kashmir,
Shalimar,
Srinagar,
191 121
Jammu and Kashmir
India
2
Division of Environmental Sciences,
S.K. University of Agricultural Sciences and Technology of Kashmir,
Shalimar,
Srinagar,
191 121
Jammu and Kashmir
India
Corresponding author Email: rfarooqlone@yahoo.co.in
DOI: http://dx.doi.org/10.12944/CWE.13.2.13
Copy the following to cite this article:
Lone F. A. Kirmani N. A. Growth and yield of Capsicum Annum Under Irrigation with Different Levels of Domestic Waste Water. Curr World Environ 2018;13(2). DOI:http://dx.doi.org/10.12944/CWE.13.2.13
Copy the following to cite this URL:
Lone F. A. Kirmani N. A. Growth and yield of Capsicum Annum Under Irrigation with Different Levels of Domestic Waste Water. Curr World Environ 2018;13(2). Available from: https://cwejournal.org/article/1086/
Download article (pdf) Citation Manager Publish History
Select type of program for download
Endnote EndNote format (Mac & Win) | |
Reference Manager Ris format (Win only) | |
Procite Ris format (Win only) | |
Medlars Format | |
RefWorks Format RefWorks format (Mac & Win) | |
BibTex Format BibTex format (Mac & Win) |
Article Publishing History
Received: | 2018-02-04 |
---|---|
Accepted: | 2018-07-25 |
Plagiarism Check: | Yes |
Reviewed by: | Gangadhar Andaluri |
Second Review by: | Rusu Teodor |
Final Approval by: | Dr. Umesh Chandra Kulshrestha |
Waste water is generated in bulk quantity from both domestic as well as industrial estates and its crude disposal is posing a tremendous threat to environment. In developing countries including India, wastewater is commonly a choice for irrigating agricultural field.1 Globally, approximately 20% of manufactured nitrogen and phosphorous is contained in domestic wastewater2 and it has been considered as low price fertilizer because of its high N, P and K content.3 Waste water being rich in organic matter and also contains appreciable amounts of macro and micro-nutrients.4 Accordingly nutrients levels of soil are expected to improve considerably using continuous irrigation with waste water.5,6,7,8,9,10,11 Thus, recycling of wastewater shall pave avenues in agricultural industry in terms of minimizing the use of inorganic fertilizers whose long-term utilization pose negative pressures on natural environment. The main objective of this experiment was to estimate the growth performance, quality parameters and yield responses of Capsicum annum var. Nishat-1 when irrigated with domestic wastewater. Moreover, effect of wastewater fertigation on the physicochemical parameters of soil and plant nutrients was studied to investigate the prospects of its safe use.
Materials and Methods
Study Area
The experimental field at FoA Wadura, SKUAST-Kashmir, was used during summer (Kharif) 2012 and 2013. The study area falls in 340 20/ 55.72”N and 740 24/ 6.26”E at an altitude of 1580 m a.s.l. Temperate climatic conditions prevail in the area with mean maximum and minimum temperature as 26.080C and 11.780C respectively. The average precipitation recorded during the experimentation was 628.4 mm and 926.2 mm in 2012 and 2013 respectively.
Experimental Design and the Crop
The different concentrations of wastewater were used along with recommended doses of fertilizers to study the growth, yield and quality of Capsicum annum var. Nishat-1. The experiment was laid in completely randomized block design and involved six treatments replicated thrice (T1: Recommended dose of fertilizers; T2: 100% Grey water; T3: 100% Black water; T4: 50% Grey water + 50% RDF; T5: 50% black water + 50% RDF; T6: 50% black water + 50% Grey water) along with an untreated control (C) irrigated with fresh water. The fertilizers N, P and K were applied as per package and practice prevailing in the university at the rates of 120, 90 and 40 (kg ha-1), as urea, DAP and MOP respectively. The crop was grown in plots measuring 1.74 × 2.3 m with 45 cm spacing between the plants and 60 cm between the rows. The grey water and black water for irrigation was obtained from the hostels of the university campus and stabilized in open tanks for 20-25 days before being put to use.
Water Analysis
Physicochemical parameters viz; pH and EC were accomplished by following the method of Piper.12 On the other hand estimation of N, P, K, alkalinity, Ca, Mg, Zn, Cu, Mn, Fe, Cd and Ni of the waste water samples were done by following the methods given by APHA.13
Soil Analysis
Composite soil samples were collected from each plot at the depth of 0-30 cm before transplantation and after harvest. Samples were oven dried, ground, sieved and analysed for pH, EC,12 total N,14 available P,15 organic carbon16 and exchangeable Ca, Mg and K using the ammonium acetate method.17 Zn, Mn, Cu, Fe, Ni and Cd were analysed by using atomic absorption spectrophotometer, after proper standardization. The soils are deep imperfectly drained, calcareous fine silty texture on nearly level slopes with loamy surface and slight flooding, the soils belong to the order of Inceptsols, with aquepts sub order Haplaquepts great group and Typic Haplaquepts sub group.
Crop Yield and Plant Tissue Analysis
Photosynthetic pigments in the foliage were estimated18 and fruit samples were harvested from each plot, oven dried for 2 days at 700C, powdered in a grinder and subjected to chemical analysis for the estimation of carbohydrate,19 protein20 and ascorbic acid contents.21 Fruit samples were also analyzed for TSS by using Refractometer. Plant nutrients were determined wet oxidation method using 3:1 ratio of HNO3: HClO4.22 Total N was determined by Kjeldahl method,14 P by spectrophotometer, K by flame photometer. Ca, Mg, Zn, Fe, Mn, Cu, Cd and Ni were estimated by atomic absorption spectrophotometer. Total weight of fruits (kg/plot) at each picking was added to obtain the total fruit yield per plot and expressed as quintals per hectare (q ha-1). Pooled data of the two years recorded during the experiment was subjected to ANOVA, followed by least significant difference (CD, p ≤ 0.05) using R software statistical package.23
Results and Discussion
Water Chemistry
Data regarding the physicochemical characteristics of black, grey and fresh water are presented in Table 1. The values were in lower levels than those permitted for upper threshold set of irrigation water and safe reuse in agriculture. According to PCB,24 pH of wastewater should range between 6.5 and 8.5 and EC should not exceed 2.25 dS/m. Our study show that both these parameters of wastewater were within range. Alkalinity, phosphate, Na, Ca, Mg, Zn, Cu, Fe, Mn and Cd were found higher in grey water compared to black and fresh water while as Cl, Fl, SO4, NH4 and K were found in higher concentration in black water. The concentration of Ni and Pb did not show any significant variation. However, all the parameters were within permissible limits.
Table 1: Physicochemical Characteristics of Irrigation Water.
Parameters
|
Waste/ Fresh Water
|
||
Black
|
Grey
|
Fresh
|
|
pH (1:2.5)
|
6.80
|
7.42
|
7.10
|
EC (dSm-1)
|
0.85
|
0.78
|
0.47
|
Alkalinity (mg/l)
|
150.2
|
180.99
|
125.13
|
Nitrate N (mg/l)
|
49.09
|
10.0
|
3.36
|
Phosphate (PO4) (mg/l)
|
3.4
|
6.21
|
0.6
|
Chloride (Cl-) (mg/l)
|
524.7
|
206.99
|
1.96
|
Fluoride (F‑ ) (mg/l)
|
0.16
|
0.14
|
0.10
|
Sulphate (mg/l)
|
1.79
|
0.84
|
0.53
|
Ammonium (mg/l)
|
169.81
|
90.22
|
2.25
|
Sodium (mg/l)
|
16.53
|
17.78
|
7.31
|
Potassium (mg/l)
|
83.92
|
17.61
|
0.51
|
Calcium (mg/l)
|
47.14
|
48.31
|
23.89
|
Magnesium (mg/l)
|
10.18
|
10.44
|
5.74
|
Zn (mg/l)
|
0.99
|
5.45
|
0.47
|
Cu (mg/l)
|
0.35
|
0.98
|
0.23
|
Fe (mg/l)
|
0.57
|
0.88
|
0.10
|
Mn (mg/l)
|
0.17
|
0.65
|
0.08
|
Cd (mg/l)
|
0.245
|
0.609
|
0.020
|
Cr (mg/l)
|
0.226
|
0.226
|
0.226
|
Pb (mg/l)
|
0.001
|
0.001
|
0.001
|
Ni (mg/l)
|
0.076
|
0.076
|
0.076
|
Physio-Chemical Characterization of the Soil
Texture of the soil was loamy and the soil pH of various treatments ranged from 6.70 to 7.56 while EC ranged from 0.20 to 1.10. It is evident form the data that various waste water treatment slightly changed the pH of the soil as also reported earlier25 (Table-2). Significant increase in EC was also detected in soil after the experiment26,27,28,29,30 which might be due to the relatively higher EC of waste water and formation of metallic salts-complexes of organic matter and heavy metals.31 Compared to initial status, the level of N and P exhibited elevated trends in various waste water treatment which can be attributed to the higher concentration of the macronutrients in grey as well as black water On the other hand lower level of Ca and Mg were observed in waste water compared to initial statues of the soil.
Table 2: Physico- chemical characteristics of the soil of capsicum annum plots after the treatments. click here to view figure |
Table 3: Effect of different concentrations of wastewater on growth parameters of Capsicum annum (Nishat -1)
Treatment |
Root length (cm) |
shoot length (cm) |
Root fresh wt (g) |
Root dry wt (g) |
Shoot fresh wt (g) |
Shoot dry wt (g) |
Leaf area (cm2 ) |
Recommended dose of fertilizers |
7.02 |
25.31 |
10.01 |
4.90 |
172.00 |
51.35 |
34.02 |
100% Grey water |
5.40 |
20.31 |
8.56 |
4.30 |
138.00 |
21.52 |
22.09 |
100% Black water |
5.22 |
20.14 |
9.56 |
4.57 |
109.00 |
23.85 |
21.60 |
50% Grey water+ 50%RDF |
7.40 |
20.15 |
10.73 |
3.45 |
126.00 |
26.00 |
26.20 |
50% black water + 50% RDF |
7.20 |
21.66 |
14.22 |
6.64 |
116.00 |
23.09 |
24.70 |
50% black water + 50% Grey water |
5.20 |
21.00 |
10.73 |
4.02 |
102.00 |
23.00 |
18.10 |
C.D (p<0.05) |
0.95 |
1.93 |
2.34 |
1.12 |
25.90 |
10.50 |
5.64 |
Crop Yield and Fruit Quality
There has been significant effect of treatments on the fresh / dry, root /shoot biomass as well as leaf area of the crop. Compared to other treatments, T1 (RDF), recorded the highest values of these parameters (Table-3) followed by T4. However, in other treatments the differences have been only marginal. The highest yield of capsicum was recorded in T1 (288.12 q ha-1) which might be due to the application of balanced doze of inorganic fertilizers and organic manures to the soil followed by 283.49 q ha-1 in T4 (50% grey water and 50%RDF ), 275.92 q ha-1 in T5( 50% black water + 50% RDF), 275.03 q ha-1 in T2 (100% grey water), 260 q ha-1 (50% black water and 50% grey water) and least of 251.96 q ha-1 in T3 ( 100% black water) (Table -4).
Table 4: Effect of different concentrations of waste water on yield parameters of Capsicum annum (Nishat -1).
Treatment
|
Fruit
No/plant
|
Fruit
length (cm)
|
Fruit diameter
(cm )
|
Fruit weight (g)
|
Yield /plant
(g)
|
Yield/plot
(kg )
|
Yield/ha (q)
|
Recommended dose of fertilizers
|
15.14
|
6.19
|
6.58
|
28.97
|
756.16
|
12.51
|
288.12
|
100% Grey water
|
14.75
|
5.01
|
5.41
|
27.67
|
703.00
|
11.35
|
270.03
|
100% Black water
|
14.08
|
5.75
|
5.08
|
27.62
|
654.94
|
10.08
|
251.96
|
50% Grey water+ 50%RDF
|
15.80
|
6.14
|
6.80
|
28.79
|
738.45
|
12.06
|
283.49
|
50% black water + 50% RDF
|
14.75
|
6.14
|
6.80
|
28.26
|
716.00
|
11.27
|
275.92
|
50% black water + 50% Grey water
|
14.41
|
5.14
|
5.75
|
27.43
|
676.51
|
11.21
|
260.00
|
C.D (p<0.05)
|
0.93
|
0.53
|
0.51
|
0.65
|
63.09
|
0.98
|
24.26
|
Table 5: Effect of different concentrations of wastewater on quality parameters of Capsicum annum (Nishat -1).
Treatment
|
TSS
(Brix)
|
Ascorbic acid
(mg/100g)
|
Chlorophyll a (mg/g tissue)
|
Chlorophyll b (mg/g tissue)
|
Total Chlorophyll (mg/g tissue)
|
Carbohydrate (%)
|
Protein (%)
|
Recommended dose of fertilizers
|
8.38
|
99.12
|
2.50
|
1.43
|
3.92
|
5.00
|
1.42
|
100% Grey water
|
9.11
|
112.13
|
2.58
|
1.78
|
3.75
|
4.92
|
1.52
|
100% Black water
|
8.52
|
114.16
|
2.72
|
1.92
|
3.65
|
4.57
|
1.32
|
50% Grey water+ 50%RDF
|
9.61
|
112.15
|
2.63
|
1.62
|
3.58
|
4.00
|
1.66
|
50% black water + 50% RDF
|
9.50
|
97.47
|
2.13
|
1.17
|
3.11
|
5.25
|
1.32
|
50% black water + 50% Grey water
|
10.00
|
97.00
|
2.30
|
1.75
|
3.17
|
6.03
|
1.71
|
C.D
(p<0.05)
|
1.13
|
6.29
|
0.40
|
0.55
|
0.10
|
0.71
|
0.15
|
Treatments
|
Major Nutrients (%)
|
Micronutrient Cations (ppm)
|
Heavy metals (ppm)
|
||||||||
N
|
P
|
K
|
Zn
|
Cu
|
Fe
|
Mn
|
Pb
|
Ni
|
Cd
|
Cr
|
|
Recommended dose of fertilizers
|
1. 69
|
0.86
|
1.07
|
19.0
|
12.5
|
60.0
|
15.22
|
0.82
|
0.22
|
0.51
|
0.86
|
100% Grey water
|
1.73
|
0.44
|
1.53
|
21.71
|
14.8
|
77.6
|
16.80
|
0.88
|
0.27
|
0.55
|
1.40
|
100% Black water
|
1.60
|
0.38
|
1.50
|
21.57
|
14.6
|
75.9
|
16.60
|
0.85
|
0.25
|
0.54
|
1.35
|
50% Grey water+ 50%RDF
|
1.89
|
0.59
|
1.62
|
23.52
|
14.4
|
73.0
|
15.8
|
0.74
|
0.23
|
0.52
|
1.32
|
50% black water + 50% RDF
|
1.79
|
0.49
|
1.55
|
22.49
|
14.2
|
72.6
|
15.5
|
0.71
|
0.21
|
0.48
|
1.27
|
50% black water + 50% Grey water
|
1.68
|
0.43
|
1.51
|
21.60
|
13.3
|
76.0
|
16.50
|
0.83
|
0.24
|
0.53
|
1.37
|
C.D (p<0.05)
|
0.35
|
0.09
|
0.27
|
1.03
|
0.85
|
4.28
|
0.98
|
0.12
|
0.02
|
NS
|
NS
|
Acknowledgments
The authors express their gratitude to J&K State Council for Science and Technology, Department of Science and Technology for providing financial assistance under which this research work was carried out.
References
- Nath KSD, Shyam S, Sharma YK. Phytotoxic effects of chromium and tannery effluent on growth and metabolismof Phaseolus mungo Roxb. Journal of Environmental Biology. 2009;30,227-234.
- Batstone D.J, Hülsen T, Mehta CM, Keller, J. Platforms for energy and nutrient recovery from domestic wastewater: a review. Chemosphere. 2015; 140: 2–11.
CrossRef - Chaw R, Reves AS. Effect of waste water on Mentha piperita and Spinacea oleraceae. Journal of Environmental Biology 2001; 51: 131-145.
- Gupta AP, NarwalRP, Antil RS. Sewer water composition and its effect on soil properties. Bioresource Technology. 1995; 65: 171-173.
CrossRef - Ramirez-Fuentese, Lucho-Constsntino, C, Escamilla-Silva, E, Dendooven, L. Characteristics and carbon and nitrogen dynamics in soil irrigated with waste water for different lengths of time. Bioresource Technology. 2002; 85: 179-187.
CrossRef - Rattan RK, Datta SP, Chhonkar PK, Suribabu, K, Singh AK. Long-term impact of irrigation with waste water effluents on heavy metal content in soils, crops and groundwater-a case study. Agriculture, Ecosystem and Environment. 2005; 109: 310-322.
CrossRef - Mahmoud EK, Ghoneim AM. Effect of polluted water on soil and plant contamination by heavy metals in El-Mahla El-Kobra, Egypt. Solid Eart. 2016; 7: 703–711.
CrossRef - Jaramillo MF, Restrepo I. Wastewater reuse in agriculture: A review about its limitations and benefits Jaramillo. Sustainability. 2017; 9: 1734.
CrossRef - arantino E, Disciglio G, Gatta G, Libutti A, Frabboni L, Gagliardi A, Tarantino A. Agroindustrial treated wastewater reuse for crop irrigation: Implication in soil fertility. Chemical Engineering Transactions. 2017; 58: 679-84.
- Santos SR, Ribeiro DP, Matos AT, Kondo MK, Araújo ED. Changes in soil chemical properties promoted by fertigation with treated sanitary wastewater. Journal of the Brazilian Association of Agricultural Engineering. 2017; 37(2):343-352.
- Gagliardi A, Disciglio G, Lonigro A, Francavilla M, Tarantino E, Giuliani MM. Irrigation with treated municipal wastewater on artichoke crop: Assessment of soil and yield heavy metal content and human risk giuseppe gatta. Water. 2018; 10 :255.
CrossRef - Piper CS. Soil and Plant Analysis. Interscience Publ. Inc., New York. 1996.
- APHA. Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association, Washington DC. 2005.
- Jackson ML. Soil Chemical Analysis, Prentice Hall of India Private Limited, New Delhi, India.1973.
- Olsen SR, Cole JV, Watanabe FS, Dean LA. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Circular 939. USDA, Washington.1954.
- Walkley A, Black IA. An examination of the Digestion method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science. 1934; 34: 29-38.
CrossRef - Hesse PR. A Text Book of Soil Chemical Analysis. John Murray Ltd. London, UK : 528. 1971.
- Hiscox JD, Isrealstam GF. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany. 1979; 57: 1332-34.
CrossRef - Dubois M, Gilles KN, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 1956; 28: 300-356.
CrossRef - Lowry OH, Rosebrought NJ, Farr AL. Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry. 1951; 193: 265-275.
- Sadasivam S, Balasubramanian T In: Practical Manual in Biochemistry. Tamil Nadu Agricultural University Coimbatore. p:14.1987.
- AOAC. Official Methods of Analysis of Association of Official Analytical Chemists, Inc. Arlington, Virginia U.S.A. 1984.
- Gomez KA, Gomez AA. Statistical Procedures for Agricultural Research. 2nd ed. A WileyInterscience Publication. John Wiley and Sons, New York.1984.
- PCB, Pollution Control Board, Andhra Pradesh. State of the Environment of Andhra Pradesh, www.appcb.org. 2003.
- Shahalam A, Abu Zahara MB, Jaradat,A. Wastewater irrigation effect on soil, crop and environment; A pilot scale study at Ibrid, Jordan. Water, Air Soil Pollution. 1998; 106: 425-445.
CrossRef - Vela SS, Dı´az-Crespo C, Navarro-Pedren˜o,J. Mataix-Beneyto, J Go´mez. I. Effect of composted sewage sludge application to soil on sweet pepper crop (Capsicum annuum var. annuum) grown under two exploitation regimes. Waste Management. 2007; 27: 1509–1518.
CrossRef - Lone FA, Qureshi N, Kirmani NA, Sidiquii, SH, Zaffar S, Shah RA. Studies on the growth performance, mineral composition and yield of bottle gourd under irrigation with grey water. Nature, Environment and Pollution Technology. 2012; 11 (2): 249-252.
- Lone FA, Kirmani NA. Sewage sludge modulates soil properties, morphophysiological characteristics and yield of raddish (Raphanus sativus) var white Round. SKUAST Journal of Research. 2018a; 20(1): 85-89.
- Lone FA, Kirmani NA. Effect of sewage sludge and inorganic fertilizers on growth performance of Capsicum annum var. Nishat-1 under temperate conditions of Kashmir. Applied Biological Research. 2018b; 20(2): 130-136.
CrossRef - Lone FA, Kirmani NA. Effect of Wastewater Fertigation on Growth Performance, Yield and Quality Parameters of Solanum melongena L. (Local Long). International Journal of Current Microbiology and Applied Sciences. 2018c; 7(3): 2138-2145.
CrossRef - Ahmed HK, Fawy HA, ES Abdel-Hady. Study of sewage sludge use in agriculture and its effect on plant and soil. Agriculture and Biology Journal of North America. 2010; 1(5): 1044-1049.
CrossRef - Khaled SB, Fathy S, Nakhlawi EL, Saleh MI, Samir G, Al-Solimani. Treated wastewater use and its effect on water conservation, vegetative yeild, yield components and water use efficiency of some vegetable crops grown under two different irrigation systems in western region, Saudi Arabia. Annual International Interdisciplinary Conference, AIIC Azores, Portugal. 2013.
- Arcadia NW, Esparza LC, Fenn LB, Ali AS, Miyamoto S, Figueroa UV, Warrick AW. Spatial variability of heavy metals in irrigated alfalfa fields in the upper Rio Grande River basin. Agricultural. Water Management. 1998; 36: 141-156.
CrossRef - Roy S, Banna LN, Mamun SA, Farukh, MA. Effects of industrial wastewater reuse for crop production: A case study in Tejgaon metropolitan area of Dhaka, Bangladesh. Journal of Bangladesh Agricultural University. 2013; 11(2): 183–188.
- Alghobar MA, Suresha S. Effect of Wastewater irrigation on growth and yield of rice Crop and uptake and accumulation of nutrient and heavy metals in Soil. Applied Ecology and Environmental Sciences. 2016; 4(3): 53-60.
- Panoras GA, Ilias KA. Irrigation with reclaimed municipal wastewater. Giachoudi Giapouli Press, Thessaloniki, Greece, 171 p. 1999.