Histochemical Analysis of Expression Pattern of Camv 35S Promoter in Transgenic Rapeseed (Brassica Napus L.)
Parissa Jonoubi1 * , Ali Hatef Salmanian2 and Amin Ravaei1
1
Department of Plant Biology, Faculty of Biological Sciences,
Kharazmi University,
Tehran,
Iran
2
Department of Plant Biotechnology,
National Institute of Genetic Engineering and Biotechnology (NIGEB),
Tehran,
Iran
http://dx.doi.org/10.12944/CWE.10.Special-Issue1.90
Promoters are considered valuable tools for biotechnology and provide great opportunities for eugenic purposes. This study aimed to investigate the expression pattern of GUS gene directed by CaMV 35S promoter in transgenic rapeseed (Brassica napus L.). The GUS gene was transferred to the rapeseed explants by Agrobacterium. The regenerated and rooted transgenic plantlets were transferred to the pots containing a mixture of soli and vermiculite. These plants underwent PCR and histochemical GUS assay. The cross-section of leaf, petiole, and stem of the transformed plants was obtained. GUS activity was observed in phloem, parenchyma, collenchyma, and supporting tissue of vascular bundles. It was also observed in cambium, endoderm, vascular ray, pith parenchyma, epidermis, and trichomes. These results showed that CaMV 35S causes the expression of transgene in various tissues differently.
Copy the following to cite this article:
Jonoubi P, Salmanian A. H, Ravaei A. Histochemical Analysis of Expression Pattern of Camv 35S Promoter in Transgenic Rapeseed (Brassica Napus L.). Special Issue of Curr World Environ 2015;10(Special Issue May 2015). DOI:http://dx.doi.org/10.12944/CWE.10.Special-Issue1.90
Copy the following to cite this URL:
Jonoubi P, Salmanian A. H, Ravaei A. Histochemical Analysis of Expression Pattern of Camv 35S Promoter in Transgenic Rapeseed (Brassica Napus L.). Special Issue of Curr World Environ 2015;10(Special Issue May 2015). Available from: http://cwejournal.org?p=680/
