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Hydroxamate Siderophore from Bacillus Sp SD12 Isolated from Iron Factory Soil

Radhakrishnan M1 * , K. J. Samshath2 and R. Balagurunathan3

1 Centre for Drug Discovery and Development, Sathyabama University, Chennai, 600 119 Tamil Nadu India

2 Department of Microbiology, Sri Sankara Arts and Science College, Kanchipuram, 631 561 Tamil Nadu India

3 Department of Microbiology, Periyar University, Salem, 636 011 Tamil Nadu India

DOI: http://dx.doi.org/10.12944/CWE.9.3.53

The present study reports siderophores production from bacteria isolated from Iron factory soil and its antifungal activity. Of the twelve morphologically different bacterial cultures isolated from iron factory soil, Kanchipuram, only two strains SD6 and SD12 showed siderophores production in Chrome Azurol S assay. Strain SD12 which showed maximum siderophores production, was selected for further studies. Siderophores produced by the strain SD12 was identified as hydroxymate type. Partial purification was carriedout by ammonium sulphate precipitation and dialysis. The molecular weight of partially purified siderophores was found to be 80KDa and it showed good antifungal activity against Fusarium solani, Aspergillus niger and Phythium sp. The potential strain was identified as Bacillus sp SD12 based on their phenotypic characteristics. Further purification and characterization of siderophores from strain SD12 pave the way for its utilization in the field of medicine and agriculture.

Siderophore; CAS assay; Bacillus; Ion factory soil; Antimicrobial

Copy the following to cite this article:

Radhakrishnan M, Samshath K. J, Balagurunathan R. Hydroxamate Siderophore from Bacillus Sp SD12 Isolated from Iron Factory Soil. Curr World Environ 2014;9 (3) DOI:http://dx.doi.org/10.12944/CWE.9.3.53

Copy the following to cite this URL:

Radhakrishnan M, Samshath K. J, Balagurunathan R. Hydroxamate Siderophore from Bacillus Sp SD12 Isolated from Iron Factory Soil. Curr World Environ 2014;9(3). Available from:http://www.cwejournal.org/?p=7721

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