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Isolation and Characterisation of Diazotrophic Bacteria from Rhizosphere of Different Rice Cultivars of South Assam, India

Folguni Laskar1 * and G.D. Sharma2

1 Department of Life Science and Bio-Informatics, Assam University, Silchar, 788 011 India

2 Department of Life Sciences, Bilaspur University, Chattisgarh, 495 009 India

DOI: http://dx.doi.org/10.12944/CWE.8.1.20

Free living heterotrophic bacteria were isolated from the rhizosphere of 10 local and cultivated varieties of rice grown in Karimganj district of South Assam. Among the 25 isolates, 11 isolates withplant growth promoting activity were identified based on phenotypic and 16S rDNA sequence analysis. The strains were identified as Shingomonasazotifigens, Pseudomonas putida, Stenotrophomonasmaltophila,Acinetobacterradioresistance, Alkaligenesfaecalis, Enterobactercloaceae subsp. dissolvens, Pantoeaagglomerans, Klebsiellapneumoneae, Achromobacterxyloxidans, Herbispirillumrubrisubalbicans and Herbispirillum sp. The efficient strains are isolated from the local varieties of rice plant. The isolate KR-23 (Sphingomonasazotifigens) was a novel bacteria reported for the first time as nitrogen fixing bacteria from India. The nitrogen fixing ability along with IAA production, ACC deaminase activity and P-solubilisation by the bacteria has shown their potential for plant-growth-promoting rhizobacteria. KR-6(Stenotrophomonasmaltophila) and KR-7(Herbispirillumrubrisubalbicans) have been reported earlier as plant pathogensbut theyhave shown a high potential for nitrogen fixing and auxin producing activity in the present study.

Nitrogen Fixation; Plant Growth Promoting Rhizobacteria; 16S rDNA Sequence; Indole-3-Acetic Acid; ACC Deaminase Activity

Copy the following to cite this article:

Laskar F, Sharma G. D. B. Isolation and Characterisation of Diazotrophic Bacteria from Rhizosphere of Different Rice Cultivars of South Assam, India. Curr World Environ 2013;8(1) DOI:http://dx.doi.org/10.12944/CWE.8.1.20

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Laskar F, Sharma G. D. B. Isolation and Characterisation of Diazotrophic Bacteria from Rhizosphere of Different Rice Cultivars of South Assam, India. Curr World Environ 2013;8(1). Available from: http://www.cwejournal.org/?p=3468

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